Abstract
Investigating the microbial diversity of Actinobacteria inhabiting the soils of the Kula-Salihli Geopark and identifying species at the genus level using 16S rRNA gene sequences are the primary goals of this work. In the literature review of this geopark located within the borders of Manisa province, no study on actinobacterial biodiversity was found. In this study, 10 different selective isolation media were used to investigate the biodiversity of Actinobacteria in the Geopark. A total of 469 Actinobacteria strains were isolated using the dilution-plate method. From these 469 strains, 34 strains were selected based on their colony morphology and pigmentation characteristics. The isolates performed phylogenetic analysis based on sequencing of the 16S ribosomal RNA gene region. The isolates were found to belong to nine different genera, including Actinomadura, Amycolatopsis, Kribbella, Micromonospora, Nocardia, Nonomuraea, Pseudonocardia, Saccharothrix and Streptomyces, according to the results of phylogenetic analysis. Five isolates have been identified as novel species as a consequence of our current study.
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