Abstract
Understanding the level and distribution of genetic diversity in African locust bean (Parkia biglobosa) would strengthen breeding and conservation programmes towards domestication and sustainable use of this species. Sixteen accessions of P. globosa were assessed for variability based on seed morphology, seed protein and DNA profiling. Significant variation in seed characteristics were observed across locations. Seed protein profiling by SDS-PAGE revealed homogeneity as most bands were found common in all accessions, indicating that the protein profiles are highly conserved. Protein profiling separated the 16 accessions into four major clusters at 0.93 similarity coefficient. Most accessions grouping into Cluster 1 had a similarity coefficient of close to 100% and were from the Derived Savanna suggesting the presence of duplicates. Accessions NH/2016/P14, NH/2016/P03 and NH/2016/P04 grouped into clusters II, III and IV; respectively. Sixteen RAPD markers generated a total of 256 bands of which 63.67% were polymorphic. Gene diversity ranged from 0.41 to 0.93 and Polymorphic Information Content (PIC) from 0.39 to 0.93. The RAPD-based dendrogram separated accessions into six groups at 0.68 similarity coefficient. Based on a polymorphic seed storage protein marker a genetically distinct accession NH/2016/P04 could be exploited for breeding purposes. The homogeneity of alleles and narrow genetic base as revealed by RAPD and SDS-PAGE analyses suggests possible loss of intraspecific genetic diversity. Thus, intensification of germplasm collections across the different agroecological zones and characterization using specific markers will give a better understanding of diversity of P. biglobosa in order to enhance selection towards conservation, breeding and sustainable utilization.
Highlights
In Sub-Saharan Africa several indigenous agroforestry systems exist containing woody species known as multipurpose trees
This study aimed to estimate the genetic diversity of P. biglobosa accessions across different agro-ecological zones in Nigeria using SDS-polyacrylamide gel electrophoresis (PAGE) and Random Amplified Polymorphic DNA (RAPD) markers
The low level of variation observed among the accessions as revealed by SDS-PAGE limits the method’s applicability to distinguish accessions
Summary
In Sub-Saharan Africa several indigenous agroforestry systems exist containing woody species known as multipurpose trees. Genetic variation of African locust bean from selected Agroecological zones in Nigeria agroforestry fruit tree species belonging to the subfamily Mimosoideae and family Fabaceae (Amusa et al, 2014; Houndonougbo et al, 2020). The seeds are an important source of plant protein and essential amino acids among rural communities who often have limited access to animal proteins due to high cost (Akin-Idowu et al, 2018; Alabi et al, 2005) and are rich in energy value, saccharose, vitamin C, lipids, carbohydrates (Orwa et al, 2010) and bioactive components such as phenolic compounds which may contribute in health promoting properties (Dedehou et al, 2016; Okoye et al, 2014). The leaves, bark, roots and flowers are used in the treatment of many diseases such as hypertension, wound healing and malaria (Dedehou et al, 2016; Ouedraogo, 1995)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
