Biodiesel production from wet microalgal biomass by direct transesterification

Abstract

The aim of this work was to optimize the production of fatty acid methyl esters (FAMEs, biodiesel) from marine Nannochloropsis gaditana wet microalgal biomass (25wt% dry biomass and 11.1wt% saponifiable lipids, SLs, of dry biomass) by direct acid catalyzed methylation of SLs in the microagal biomass and extraction of FAMEs with hexane. The best FAME yield was attainted with a methanol/SL ratio of 171.1mL/g, an acetyl chloride (catalyst) concentration of 5% (v/v), 100°C and a reaction time of 105min. In these conditions 100% of SLs were transformed to FAMEs. Similar yields were obtained at lower temperatures and higher reaction times. The pretreatment of wet biomass by homogenization did not improve the above results. Finally, the FAME purity was increased from 74.5 to 82.7wt% by a treatment of adsorption with bentonite.