Biodiesel Co-Product enhances microbial stability and beneficial microbial communities along a gradient of soil water content

Abstract

Biodiesel Co-Product (BCP) is a complex carbonaceous liquid formed during the commercial production of biodiesel. Previously, BCP was shown to decrease nitrogen (N) leaching from the soil, but the effects of BCP on the diversity, composition, and structure of soil microbial communities are not well understood. Here, we applied 1.5 mg BCP-C to acidic soil (pH 3.5) at a range of different water contents (from 40 % to 100 % water holding capacity) to investigate the interactions between BCP and increasing water holding capacity on the diversity, composition, and interactions of soil microbial communities. Distance-based multivariate linear model (DistLM) and non-metric multidimensional scaling (NMDS) analyses showed that BCP caused larger changes in fungal than bacterial communities, while soil water content had a greater effect on bacterial communities relative to fungal communities. Co-occurrence network analyses indicated that BCP amendment produced more robust and complex bacterial networks and more stable fungal ones. BCP significantly increased the OTU numbers of beneficial microbes (e.g., Trichoderma spp.) in all water contents, with fewer OTU numbers of putative pathogenetic species (Fusarium spp. and Aspergillus spp.). These findings indicate that BCP addition may be conducive to the health and stability of soil ecosystems.