Abstract
Biodegradation of light and high-viscosity oils by hydrocarbon-oxidizing microflora has been studied. Microflora was isolated from the formation waters recovered from West Siberian and “White Tiger” (Vietnam) oil fields. To activate microorganisms one used the solution of IKhN-KA system containing a multi-component nitrogenous nutrient substrate. Under the condition of active development of microorganisms during 5 days of biodegradation the concentration of n-alkanes C10-C32 in light oils decreased by 70-85 % and that of viscous oil – by 86-93 %. Destruction of mono-aromatic compounds accounted for 55-65 % and that of aromatic compounds of naphthalene series – 70-90 %. The content of methyl- and<br />trimethyl phenanthrenes decreased 3 times. Therefore a degree of oil destruction depends on nutrient substrates, which stimulate biochemical processes of vital activity.
Highlights
The ability of microorganisms for hydrocarbon biodegradation is used to develop commercial biotechnologies, including biotechnologies for enhanced oil recovery.Spontaneous biodegradation of hydrocarbons is constantly proceeding in nature and depends on many factors
Oils recovered from West Siberian oil fields are light and low viscous under the formation conditions
Biodegradation of n-alkanes in light oils recovered from West Siberian oils were accompanied with the destruction of mono-aromatic compounds, the concentration of which decreased by 50 %
Summary
The ability of microorganisms for hydrocarbon biodegradation is used to develop commercial biotechnologies, including biotechnologies for enhanced oil recovery.Spontaneous biodegradation of hydrocarbons is constantly proceeding in nature and depends on many factors. To stimulate the growth and fermentative activity of microflora we used 0.1 % solution of oil-displacing IKhN-KA system as a nitrogenous substrate. With the injection of 0.1 % solution of IKhN-KA system, which was preliminary autoclaved, into a cultural medium the number of microflora increased by 3-6 orders and the rate of oil hydrocarbon destruction increased up to 35 mg/L in a day.
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