BiodEnz:A database of biodegrading enzymes.

Shobana Sugumar,Berla Thangam

doi:10.6026/97320630008040

Abstract

Azo dyes, which re characterized by azo bonds, are a predominant class of colorants used in tattooing, cosmetics, foods, textile and consumer products. Laccases (EC 1.10.3.2), lignin peroxidases (EC 1.11.1.14) , Azo reductases (EC 1.7.1.6) of different micro organisms are mainly useful for the development of biodegradation systems as they catalyse reductive cleavage of azo groups (-N=N-) . Laccases have very broad substrate specificity with respect to the electron donor and is capable of oxidizing phenols and aromatic amines. Azoreductase belongs to the family of oxidoreductases, acting on other nitrogenous compounds as donors with NAD+ or NADP+ as acceptor. Lignin peroxidase enzymes are highly non-specific and are well reported to decolourize various dyes We have developed BiodEnz database by collecting information like strains that produce particular enzymes, azo dyes that are degraded , substrate specificity, molecular weight, the optimum temperature and pH, sequence data of the above enzymes ,as the most effective inoculants used for bioremediation are able to degrade dyes over a broad concentration range, tolerate a range of environmental conditions of temperature, pH, and activity of the enzymes. The database can be searched by using a user friendly web interface. The database is available for free at http://www.biodenzdatabase.in.

Highlights

Azo dyes which represent about one-half of all dyes in common use are widely used by the textile, leather, cosmetics, food coloring and paper production industries
Bioremediation of azo dyes in textile waste effluents by bacteria or fungi is a very promising area of study because of the relatively low expense involved.Bioremediation includes biodegradation and biotransformation, with a goal to open access mineralize hazardous contaminants in the environment. This is fulfilled by the azodye degrading enzymes like laccases,azoreductases,lignin peroxidases and some more which are vastly available in the variety of micro organisms like fungi, algae, bacteria
Azoreductase catalyzes the reductive cleavage of azo linkages in benzidine based dyes and other compounds containing an azo bond to produce aromatic amines.many bacterial strains possess many unspecific cytoplasmic enzymes which act as azoreductases

Summary

Introduction

Azo dyes which represent about one-half of all dyes in common use are widely used by the textile, leather, cosmetics, food coloring and paper production industries. Azodyes are recalcitrant to biodegradation due to their complex structures and xenobiotic nature, and typically require an anaerobic–aerobic process to achieve complete degradation.

Results

Conclusion