Biodegradation of Uranium−Citrate Complexes: Implications for Extraction of Uranium from Soils

Abstract

Citrate is often used as a complexing agent to mobilize sorbed and precipitated uranium in both in situ and ex situ extraction of soils and nuclear reactor components. The biodegradability of U−citrate complexes is an important control over the potential migration of residual uranium after the extraction process is complete. In solutions buffered at pH 6−7, limited biodegradation of citrate is observed within 10 days with initial U:citrate molar ratios ranging from 1:2 to 1:8; however, over 99% of the citrate is biodegraded rapidly at pH 8−9. The increase of pH may have shifted the equilibrium speciation of uranium from (UO2−citrate)22- to (UO2)3(OH)71- and, consequently, raised the bioavailability of citrate. At pH 6−7, a significant amount of uranium is also observed to associate with biomass, whereas only a negligible amount is observed at pH 8−9. Our experimental results suggest that the residual concentration of uranium−citrate complexes left in the treated soils can be reduced rapidly if the soil wa...