Abstract
Tribenuron methyl (TBM) is a member of the sulfonylurea herbicide family and is widely used in weed control. Due to its phytotoxicity to rotating-crops, concerns on TBM-pollution to soil have been raised. In this study, experimental results indicated that microbial activity played a key role in TBM removal from polluted soil. Twenty-six bacterial strains were isolated and their degradation of TBM was evaluated. Serratia sp. strain BW30 was selected and subjected to further investigation on its degradative mechanism. TBM degradation by strain BW30 was dependent on glucose that was converted into lactic or oxalic acids. HPLC–MS analysis revealed two end-products from TBM degradation, and they were identical to the products from TBM acidohydrolysis. Based on this observation, it is proposed that microbe-mediated acidohydrolysis of TBM was involved in TBM degradation in soil, and possible application of this observation in bioremediation of TBM-polluted soil is discussed.
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