Biodegradation of total petroleum hydrocarbons (TPH) in highly contaminated soils by natural attenuation and bioaugmentation

Abstract

Bioremediation is an emerging and sustainable technique that can either occur naturally or be enhanced by introducing nutrients or bacteria able to degrade specific contaminants. In this study, the efficiencies of natural attenuation with nutrients, and bioaugmentation with nutrients and a consortium of five exogenous bacteria, were evaluated for total petroleum hydrocarbon (TPH) degradation in five highly contaminated soils from China, and Kuwait. The bioaugmentation treatment exhibited better efficiencies than the natural attenuation, and reached 48.10% of TPH degradation with a half-life of 41.76 d. The addition of exogenous bacteria also increased the removal of TPH in the highest contaminated soil sample. The concentration of TPH in that soil was reduced from 236, 500 mg kg−1 of dry soil to 176, 566 mg kg−1 of dry soil in 40 d, which was equivalent to 25.4% degradation of TPH. The degradation rate (1501.8 mg kg−1d−1 of TPH) was higher than those reported in previous studies with a lower concentration of TPH. The bioaugmented strains could withstand high concentrations of TPH and thrive in five different types of soils. Consequently, these strains can be used to remediate soils that are heavily contaminated with petroleum hydrocarbons.