Biodegradation of the endocrine disrupter 4-tert-octylphenol by the yeast strain Candida rugopelliculosa RRKY5 via phenolic ring hydroxylation and alkyl chain oxidation pathways

Abstract

4-(1,1,3,3-tetramethylbutane)-phenol (4-tert-OP) is one of the most prevalent endocrine disrupting pollutants. Information about bioremediation of 4-tert-OP remains limited, and no study has been reported on the mechanism of 4-tert-OP degradation by yeasts. The yeast Candida rugopelliculosa RRKY5 was proved to be able to utilize 4-methylphenol, bisphenol A, 4-ethylphenol, 4-tert-butylphenol, 4-tert-OP, 4-tert-nonylphenol, isooctane, and phenol under aerobic conditions. The optimum conditions for 4-tert-OP degradation were 30°C, pH 5.0, and an initial 4-tert-OP concentration of 30mgL−1; the maximum biodegradation rate constant was 0.107d−1, equivalent to a minimum half-life of 9.6d. Scanning electron microscopy revealed formation of arthroconidia when cells were grown in the presence of 4-tert-OP, whereas the cells remained in the budding form without 4-tert-OP. Identification of the 4-tert-OP degradation metabolites using liquid chromatography–hybrid mass spectrometry revealed three different mechanisms via both branched alkyl side chain and aromatic ring cleavage pathways.