Abstract
This project is part of a major project (PI, Dr. Harvey Bolton, Jr. at PNNL) regarding plutonium mobility in the subsurface. Ethylenediaminetetraacetate (EDTA) is a common chelating agent that can increase the mobility of radionuclides and heavy metals in groundwater. Biodegradation of EDTA decreases the enhanced mobility. The overall objective is to understand how microbial degradation affects Plutonium-EDTA transport in the environment, and the specific objective of this component is to understand how microorganisms degrade EDTA. Over the past two years, significant progress has been made to the understanding on how EDTA is degraded by an EDTA-degrading bacterium BNC1. The characterization of EDTA and nitrilotriacetate (NTA) transport into BNC1 cells is summarized here. The uptake is the limiting step in EDTA and NTA degradation. The objectives of near-term research are described. We are making progress as projected in the proposal.
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