Abstract
Background: Oil spills are considered as one of the critical problems which cause a decline in environmental health. Currently the biological solutions have become more familiar to remove hazardous substances from the environment. Methods: Soil samples were collected from petrol bunks and automobile workshops at Madurai and used for the isolation of fungi. The isolated fungus was identified as Penicillium sp. using lacto phenol cotton blue staining method and cultural characteristics. The efficiency of the fungal strain on the degradation of different concentrations of petrol was studied using 2.5, 5, 7.5 and 10% of petrol in minimal medium. Results: The parameters, pH, optical density and CO released were determined. HPLC analysis exhibited a difference in the pattern of peaks between control and the 2 treated sample confirming petrol degradation. Conclusion: The ability of Penicillium sp. to tolerate oil pollutants and grow on them, suggest that it can be employed as bioremediation agent and can be used in restoring the ecosystem when contaminated by oil.
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