Abstract
The use of pesticides like Chlorpyrifos in agricultural soil is the primary reason for the pollution of aquatic and terrestrial environments. Today the most effective method used for bioremediation are by using microbes. Different pesticide degrading bacteria were isolated and identified by the mean of cultural, biochemical tests and which is further identified and confirmed by 16S RNA sequencing method. The most potent strain S-1 growth in mineral salt medium supplemented with Chlorpyrifos as sole source of carbon (50 to 1000 ug/ml) its optical density was measured at 600 nm. The bacterial growth is optimised on the parameter of different physiochemical condition were. The result showed that S. aureus shows maximum growth on 12th day. The HPLC analysis was also done for calculating the residual percentage of Chlorpyrifos after 12 days incubation which showed that S. aureus was able to degrade 99% of the pesticide of the 1000 ug/ml CP concentration in the MSM. The results of this research shows that the isolated bacteria have the potential to be used in bioremediation of Chlorpyrifos contaminated soil and water ecosystems.
Highlights
The 70% of total population of India is dependent on the agricultural primarily which is the maximum portion of the country’s economy[1]
Bacteria with the capability to utilise the pesticide organophosphate have been isolated and characterized from the soil all over the world[3], Pseudomonas aeruginosa isolated from the soil is the gram negative bacteria and is the most common bacteria with the potential to degrade Chlorpyrifos[4]
The bacterial cultures were isolated on by the help of MSM containing CP in different concentration (50 to 1000 ug/ml). 35 different bacterial cultures were obtained from different soil sample
Summary
The 70% of total population of India is dependent on the agricultural primarily which is the maximum portion of the country’s economy[1]. The extensive use of such pesticides results in the accumulation of pesticide in our atmosphere Many of these pesticides can persist in the soil and they can contaminate the surface and the ground-water. The Organophosphate (OP) insecticides like Chlorpyrifos insecticide is widely used and can lead to contamination of soil and water bodies. This pesticide is having a broadspectrum range and it is extensively used in the prevention of agricultural pests and it is moderately toxic insecticide. The main objectives of this present study are to isolate the contaminated soil bacterial strains and further to characterize its Chlorpyrifos degradation potential in the MSM consisting of Chlorpyrifos as an only source of carbon in different concentrations and other environmental and chemical factors
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