Biodegradation of penicillin G from industrial bacteria residue by immobilized cells of Paracoccus sp. KDSPL-02 through continuous expanded bed adsorption bioreactor

Peng Wang,Shouxin Liu,Chen Shen,Xiaochun Wang,Jinfeng Guo,Luwei Li

doi:10.1186/s13036-020-0229-5

Abstract

BackgroundAn efficient biodegradation-strengthening approach was developed to improve penicillin G degradation from industrial bacterial residue in an expanded bed adsorption bioreactor (EBAB) is reported in this paper.ResultsParacoccus sp. strain KDSPL-02 was isolated based on its ability to use penicillin G as the sole carbon and nitrogen source. Strain identification was based on analyses of morphology, physio-biochemical characteristics, and 16S rDNA sequences. The effects of temperature, pH, PVA-sodium alginate concentration, calcium chloride concentration and initial penicillin G concentration were investigated. Repeated operations of immobilized cells with EBAB, At initial penicillin concentrations below 2.0 g L− 1, the continuous mode could reach more than 20 times, and the degradation rate reached 100%.ConclusionsThe present study suggests that the EBAB system can be utilized for the simple and economical biodegradation of penicillin G from industrial bacterial residue.

Highlights

In recent years, biomass resources, as a kind of renewable energy, have brought benefited to mankind
Strain identification was based on analyses of morphology, physio-biochemical characteristics, and 16S rDNA sequences
A pilot study showed that KDSPL-02, without any treatment, exhibited the highest penicillin G degradation ability

Summary

Introduction

Biomass resources, as a kind of renewable energy, have brought benefited to mankind. Industrial bacterial residue from pharmaceutical industries has recently become widely used in some countries as fodder, which is rich in proteins and carbohydrates and used in animal husbandry [8]. The typical antibiotic penicillin G from penicillin fermentation fungi residue (PFFR) may accumulate in the natural environments, in places such as soil, sediments, surface water, and groundwater, and may enter the food chain and the bodies of PFFR is regarded as a hazardous solid waste that is disposed via approved methods, such ad landfills, incineration, anaerobic treatment, or composting. The waste mycelium contains large amounts of proteins, carbohydrates, and cellulose. An efficient biodegradation-strengthening approach was developed to improve penicillin G degradation from industrial bacterial residue in an expanded bed adsorption bioreactor (EBAB) is reported in this paper

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Results

Conclusion