Biodegradation of olive mill wastewater phenolic compounds in a thermophilic anaerobic upflow packed bed reactor and assessment of their toxicity in digester effluents

Abstract

The extent of phenolic compounds’ biodegradation was assessed utilizing un-treated olive mill wastewater (OMWW) fed to a high-rate thermophilic (55 οC) anaerobic upflow packed bed reactor (UPBR) and digester effluents (DEs) collected in different hydraulic retention times (HRTs) under steady-state operating conditions. In parallel, the toxicity of each sample was evaluated by performing the microbiotest Thamnotoxkit F™. The outcomes indicate complete biodegradation of 6 phenolic compounds-vanillic acid (VA), caffeic acid (CA), syringic acid (SA), o-coumaric acid (o-CA), oleuropein (OLEU), 4-ethylphenol (4-EP)-and notable removals of hydroxytyrosol (HT) and tyrosol (TYR), reaching up to 94.87 ± 0.04% and 93.92 ± 0.33%, respectively. 4-hydroxybenzoic acid (PHBA), p-coumaric acid (p-CA) and 3,4-dihydroxybenzoic acid (DBA) were recognized as the most recalcitrant and persistent compounds in the anaerobic effluents, being capable of modulating the toxic potential of DEs.