Abstract

At present, the pollution of microplastic directly threatens ecology, food safety and even human health. Polyethylene terephthalate (PET) is one of the most common of microplastics. In this study, the micro-size PET particles were employed as analog of microplastic. The engineered strain, which can growth with PET as sole carbon source, was used as biocatalyst for biodegradation of PET particles. A combinatorial processing based on whole-cell biocatalysts was constructed for biodegradation of PET. Compared with enzymes, the products can be used by strain growth and do not accumulated in culture solution. Thus, feedback inhibition of products can be avoided. When PET was treated with the alkaline strain under high pH conditions, the product concentration was higher and the size of PET particles decreased dramatically than that of the biocatalyst under neutral conditions. This shows that the method of combined processing of alkali and organisms is more efficient for biodegradation of PET. The novel approach of combinatorial processing of PET based on whole-cell biocatalysis provides an attractive avenue for the biodegradation of micplastics.

Highlights

  • In recent years, the pollution of microplastic has raised increasing concerns worldwide and is an emerging research area [1]

  • The results further indicate that the biodegradation of Polyethylene terephthalate (PET) under alkaline conditions is the hydrolysis under the action of alkali, but the result of the combination of biodegradation and alkali hydrolysis

  • In case of bacterial whole-cell biodegradation of PET, the products can be used by strain growth and do not accumulated in culture solution

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Summary

Introduction

The pollution of microplastic has raised increasing concerns worldwide and is an emerging research area [1]. Microplastics were defined as plastic particles

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