Abstract
A halotolerant bacterial consortium capable of degrading di-(2-ethylhexyl) phthalate (DEHP) was enriched from activated sludge. Community analysis revealed that LF contained seven families and seven genera of bacteria. The predominant species was Gordonia sp. (54.93%), Rhodococcus. sp. (9.92%) and Achromobacter sp. (8.47%). The consortium could degrade 93.84% of 1000 mg/l DEHP after 48 h incubation. The optimal temperature and pH for LF to degrade DEHP were 30 °C and 6.0, respectively. LF degraded more than 91% of DEHP with salt concentrations ranging from 0–3%. The inoculum size had great effects on DEHP degradation (incubation time < 24h). LF could degrade high concentrations of DEHP (from 100 to 2000 mg/l) with the degradation ratio above 92% after 72 h incubation. Kinetics analysis revealed that the degradation of DEHP by LF was best fitted by the first-order kinetics when the initial concentration ranged from 100 to 2000 mg/l. The main intermediates (2-ethylhexyl pentyl phthalate, butyl (2-ethylhexyl) phthalate (BEHP), mono-ethylhexyl phthalate (MEHP), mono-hexyl phthalate (MHP), mono-butyl phthalate (MBP)) in DEHP degradation process were identified using gas chromatography–mass spectrometry (GC-MS), and a new complex biochemical pathway was proposed. Furthermore, LF could also degrade dimethyl phthalate (DMP), diethyl phthalate (DEP), di-n-butyl phthalate (DBP), di-n-octyl phthalate (DOP) and phthalic acid (PA).
Highlights
Phthalic acid esters (PAEs) is a group of teratogenicity, carcinogenicity, mutagenicity and endocrine disruption compounds, which are widely used in PVC plasticizer to enhance their plasticity and flexibility [1,2,3]
A variety of bacterial strains capable of degrading di-(2-ethylhexyl) phthalate (DEHP) have been isolated from the environment, such as Acinetobacter sp. [19], Microbacterium sp. [20], Bacillus sp. [21], Gordonia sp. [22], Pseudoxanthomonas sp. [23] etc
Most of these researches focused on degradation of DEHP by pure bacterial strains, and little attention has been paid to DEHP degradation by bacteria consortium or bacteria combination
Summary
Phthalic acid esters (PAEs) is a group of teratogenicity, carcinogenicity, mutagenicity and endocrine disruption compounds, which are widely used in PVC plasticizer to enhance their plasticity and flexibility [1,2,3]. Technology) in University of Henan Province (16IRTSTHN012)
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