Biodegradation of dairy wastes using crude enzymatic extract of Yarrowia lipolytica ATCC 9773

Arnulfo Tarón Dunoyer,Rafael Emilio González Cuello,Rosangela Perez Salinas

doi:10.4136/ambi-agua.2448

Arnulfo Tarón Dunoyer, Rafael Emilio González Cuello + Show 1 more

Open Access

https://doi.org/10.4136/ambi-agua.2448

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Abstract

Effluents generated by the food industry have become a serious environmental concern. Bioremediation is a biological process developed as an alternative for the treatment of contaminated areas. In current research, the biodegradation of fat, Biochemical Oxygen Demand (BOD5), Chemical Oxygen Demand (COD) and total solids were evaluated in dairy waste employing enzymatic extract of Yarrowia lipolytica ATCC 9773 as biological agents. All the variables were determined following the specifications of the Standard Methods of the American Water Works Association. Enzymatic extract of Y. lipolytica at different concentrations (8, 12 and 16.0%) was used in a fermentative medium at two pHs (5.0 and 6.5) for 32 h. The highest percentages (%) of fat (82.88), BOD (43.32), COD (44.3) and total solids (13.58) removal were obtained using an inoculum concentration of 16% at pH 5.0 for 32 h of fermentation. These results may have industrial relevance for the reduction of contamination of industrial effluents with high levels of fat and other contaminants.

Highlights

The food industry is a sector with a high incidence of environmental pollution
Growth of Y. lipolytica The growth curve of Y. lipolytica ATCC 9773 under experimental conditions is illustrated in Figure 2a y 2b
The study showed that fatty effluent from the dairy industry contains high levels of BOD5, Chemical Oxygen Demand (COD), total solids and fat

Summary

Introduction

The food industry is a sector with a high incidence of environmental pollution. Different industries such as dairy, meat and vegetable oil refining produce large amounts of wastewater. The high oil content of these increase the spoilage of some ecosystems (Porwal et al, 2015). Dairy wastes are pollutants that affect the environment when they are discarded without adequate treatment (Liu et al, 2015b). Fatty effluents have been released to the environment without previous treatment (Kumari et al, 2017; Tarón-Dunoyer et al, 2017), which affects public health and environmental sustainability. Considering the aforementioned, the treatment of fatty effluents is an economic and hygienic requirement

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Conclusion