Biodegradation of copper (II) ions by Klebsiella sp. 3S1 isolated from the eutrophicated water of Dal lake in Srinagar Kashmir (India)

Abstract

ABSTRACT Water contamination by heavy metals now adversely affects almost every country. The use of microbes to clean up polluted water bodies has recently sparked a lot of debate. The copper (II)-resistant bacterium, identified as Klebsiella species 3S1, was discovered in the waters of Dal Lake Srinagar (India). The roles of pH, contact time, and initial copper (II) concentration were evaluated in the biosorption of copper (II) by Klebsiella 3S1 species. The Langmuir isotherm fitted showed the maximum biosorption capacities to be of the order of 61.73 ± 1.13, 70.13 ± 0.16, and 102.57 ± 1.13 at the respective temperatures of 293 K, 300 K, and 312 K. For the biosorption kinetics, pseudo-second-order model was found to be best fitted. SEM-EDX evidenced the copper (II) biosorption, was discovered to be linked to the cell wall structures of Klebsiella 3S1 bacteria. FTIR spectroscopy revealed that the OH, NH, COOH, amino, and hydroxyl groups were the sites of stretching and copper (II) binding. The thermodynamic study revealed that the biosorption of copper (II) by Klebsiella 3S1 is endothermic and spontaneous. This present study indicated the biosorption potential of Klebsiella species 3S1 biomaterial towards the bioremediation of copper (II)-contaminated waste water.