Biodegradation of chlortetracycline by Bacillus cereus LZ01: Performance, degradative pathway and possible genes involved

Abstract

Microbial degradation of chlortetracycline (CTC) is an effective bioremediation method. In the present study, an enrichment technique was used to isolate a Bacillus cereus LZ01 strain capable of effectively degrading CTC from cattle manure. Response surface methodology was used to identify optimized conditions under which strain LZ01 was able to achieve maximal CTC removal (83.58%): temperature of 35.77 °C, solution pH of 7.59, CTC concentration of 57.72 mg/L and microbial inoculum of 0.98%. The antibacterial effect of CTC degradation products on Escherichia coli was investigated by the disk diffusion test, revealing that the products by LZ01 degradation of CTC exhibited lower toxicity than parent compound. Shake flask batch experiments showed that the biodegradation of CTC was a synergistic effect of intracellular and extracellular enzymes, and intracellular enzyme had a better degradation effect on CTC (77.56%). Whole genome sequencing revealed that genes associated with ring-opening hydrolysis, demethylation, deamination and dehydrogenation in strain LZ01 may be involved in the biodegradation of CTC. Subsequent seven possible biodegradation products were identified by LC-MS analyses, and the biodegradation pathways were proposed. Overall, this study provides a theoretical foundation for the characterization and mechanism of CTC degradation in the environment by Bacillus cereus LZ01.