Abstract
Laccase enzyme obtained from Micrococcus luteus was exploited for biodegradation of azo dyes. The enzyme was characterized for optimum growth conditions at pH 7.0, temperature at 37 °C and incubation duration of 72 h. The molecular weight of the enzyme was found to be 63 kDa determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. Application of enzyme on the two dyes namely C.I. Acid Black 52 and C.I. Acid Blue 113 resulted in biodegradation rate of 92.2 and 94.5 %, respectively. UV–Vis spectra analysis indicated the absence of peaks in the visible region of the experimental samples confirmed the complete biodegradation of dye samples. Further, Fourier transform-infrared spectroscopy results showed the transformation of N=N into N2 or NH3 and then into biomass. Intermediate products of the biodegraded dye sample were analyzed by mass-electrospray ionization spectra. The reduction in BOD and COD values were found to be 93.2, 92.5 and 92.0, 88.7 %, respectively, for the above dyes.
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