Abstract
Pseudomonas stutzeri strain 9 was isolated from petroleum-contaminated soil. The main purpose of this study was to investigate how the long-term contact of this strain with diesel oil influences its surface and biodegradation properties. The experiments showed that the tested strain was able to degrade aromatic alkyl derivatives (butylbenzene, sec-butylbenzene, tert-butylbenzene and isobutylbenzene) and that the storage conditions had an influence on the cell surface properties. Also greater agglomeration of the cells was observed in the scanning electron microscope (SEM) micrographs and confirmed in particle size distribution results. The results also indicated that the addition of rhamnolipids to the hydrocarbons led to modification of the surface properties of P. stutzeri strain 9, which could be observed in the zeta potential and hydrophobicity values.
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