Abstract
Extensive use of pesticides has led to the contamination of ecosystem. Therefore, it is important to isolate potential new pesticide-degrading bacteria. For the biodegradation of α-endosulfan and α-cypermethrin, a new bacterium was isolated from the body microflora of grasshopper (Poecilimon tauricola). Based on biochemical, morphological, and 16S rRNA sequence analysis, the isolated strain B7 was identified as Acinetobacter schindleri. This bacterial strain was screened for its α-cypermethrin and α-endosulfan degrading potential with minimal salt medium (MSM) and non-sulfur medium (NSM), respectively. When glucose was added to non-sulfur medium containing α-endosulfan (100mg/L) and minimal salt medium containing α-cypermethrin (100mg/L), both pesticide degradation and bacterial growth were increased. Acinetobacter schindleri B7 was able to degrade 67.31% of α-endosulfan and 68.4% of α-cypermethrin within 10days. The degradation products of pesticides were determined by HPLC. As a result, A. schindleri, a Gram-negative bacterium, can inevitably be used in the biological treatment of environments exposed to pesticides.
Highlights
Pesticides are used against pests in order to obtain more products and their use is increasing day by day
Five bacteria capable of breaking down the α-endosulfan and/or α-cypermethrin were successfully isolated from nonsulfur medium (NSM)-agar or minimal salt medium (MSM)-agar plates (Table 2)
According to biochemical test and physical properties, α-endosulfan degrading isolates were identified as Pseudomonas aeruginosa B5, Bacillus megaterium B9, Acinetobacter schindleri B7 and α-cypermethrin degrading isolates were identified as Acinetobacter johnsonii B6, Acinetobacter schindleri B7 and Brevibacillus parabrevis B12
Summary
Pesticides are used against pests in order to obtain more products and their use is increasing day by day These chemicals, which are toxic to the environment, are widely used for the protection of stored products outside agricultural areas, against insects and weeds damaging forest trees (Jiang et al 2019; Gao et al 2020). According to their chemical structure, the most important pesticide groups are carbamates, organochlorinates, organophosphorus and synthetic pyrethroids. Endosulfan and disintegration products have been identified in atmospheres, soil, food products, groundwater and surface waters It can remain intact in nature for a long time and threatens the environment and public health. They have negative effects on the environment and human health due to their high rate of use (Gur et al 2014; Aguila-Torres et al 2020)
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