Abstract
The possibility to use a suspended tridimensional matrix as scaffolding for re-epithelialization of in vitro cutaneous wounds was investigated with the aid of a human in vitro wound healing model based on viable full thickness skin. Macroporous gelatin microcarriers, CultiSpher-S, were applied to in vitro wounds and cultured for 21 days. Tissue sections showed incorporation of wound edge keratinocytes into the microcarriers and thicker neoepidermis in wounds treated with microcarriers. Thickness of the neoepidermis was measured digitally, using immunohistochemical staining of keratins as epithelial demarcation. Air-lifting of wounds enhanced stratification in control wounds as well as wounds with CultiSpher-S. Immunohistochemical staining revealed expression of keratin 5, keratin 10, and laminin 5 in the neoepidermal component. We conclude that the CultiSpher-S microcarriers can function as tissue guiding scaffold for re-epithelialization of cutaneous wounds.
Highlights
The use of scaffolds for dermal regeneration in combination with cultivated keratinocytes is well accepted [1,2]
We investigated the effects of providing resident cells in cutaneous in vitro wounds a porous scaffold in the form of CultiSpher-S gelatin microcarriers
We utilized an in vitro wound healing model based on viable human skin in culture
Summary
The use of scaffolds for dermal regeneration in combination with cultivated keratinocytes is well accepted [1,2]. The desired qualities for a provisional wound healing matrix include biocompatibility, durability, a biomimetic surface for resident cells and support for tissue regeneration [4]. Most of these products are of a static nature meaning they must be produced in exactly the shape of the defect which is often clinically inefficient. This motivates the introduction of a malleable scaffold that is optimized for culture of human cells, biodegradable without toxic residues and that stabilizes when applied to a defect. Culturing of cells on microcarriers has primarily been a method for high-density cell expansion [5] and as a delivery vehicle for transplantation
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