Biodegradable 3D Printed Scaffolds of Modified Poly (Trimethylene Carbonate) Composite Materials with Poly (L-Lactic Acid) and Hydroxyapatite for Bone Regeneration.

Honglei Kang,Feng Li,Zhiwei Liu,Xudong Jiang,Guoping Yan,Fan Liu

doi:10.3390/nano11123215

Abstract

Biodegradable scaffolds based on biomedical polymeric materials have attracted wide interest in bone transplantation for clinical treatment for bone defects without a second operation. The composite materials of poly(trimethylene carbonate), poly(L-lactic acid), and hydroxyapatite (PTMC/PLA/HA and PTMC/HA) were prepared by the modification and blending of PTMC with PLA and HA, respectively. The PTMC/PLA/HA and PTMC/HA scaffolds were further prepared by additive manufacturing using the biological 3D printing method using the PTMC/PLA/HA and PTMC/HA composite materials, respectively. These scaffolds were also characterized by Fourier transform infrared spectroscopy (FT-IR), gel permeation chromatography (GPC), automatic contact-angle, scanning electronic micrographs (SEM), diffraction of X-rays (XRD), differential scanning calorimetry (DSC), and thermogravimetry (TG). Subsequently, their properties, such as mechanical, biodegradation, cell cytotoxicity, cell compatibility in vitro, and proliferation/differentiation assay in vivo, were also investigated. Experiment results indicated that PTMC/PLA/HA and PTMC/HA scaffolds possessed low toxicity, good biodegradability, and good biocompatibility and then enhanced the cell multiplication ability of osteoblast cells (MC3T3-E1). Moreover, PTMC/PLA/HA and PTMC/HA scaffolds enhanced the adhesion and proliferation of MC3T3-E1 cells and enabled the bone cell proliferation and induction of bone tissue formation. Therefore, these composite materials can be used as potential biomaterials for bone repatriation and tissue engineering.

Highlights

Academic Editors: David Jenkins and School of Materials Science and Engineering, Wuhan Institute of Technology, Wuhan 430205, China; Department of Orthopaedics, Tongji Medical College, Huazhong University of Science and Technology, These authors contributed to this work
The biodegradable poly(trimethyl carbonate) (PTMC)/HA and PTMC/poly(L-lactic acid) (PLA)/HA scaffolds were produced by the blending and further 3D printing of composite materials based on PTMC, PLA, and HA
In the PTMC/PLA/HA scaffolds, PTMC was chosen as a soft polymer segment, PLA was used as a rigid polymer segment, and HA was used as a reinforcing filler

Summary

Introduction

The PTMC/PLA/HA and PTMC/HA scaffolds were further prepared by additive manufacturing using the biological 3D printing method using the PTMC/PLA/HA and PTMC/HA composite materials, respectively These scaffolds were characterized by Fourier transform infrared spectroscopy (FT-IR), gel permeation chromatography (GPC), automatic contactangle, scanning electronic micrographs (SEM), diffraction of X-rays (XRD), differential scanning calorimetry (DSC), and thermogravimetry (TG). PTMC/PLA/HA and PTMC/HA scaffolds enhanced the adhesion and proliferation of MC3T3-E1 cells and enabled the bone cell proliferation and induction of bone tissue formation. These composite materials can be used as potential biomaterials for bone repatriation and tissue engineering. The development of novel biodegradable scaffolds based on biomedical polymeric materials for bone transplantation has become a current research hot spot [7,8,9]

Methods

Results

Conclusion