Abstract
The discharge of textile azo dyes to the environment is an issue of health concern and can harm especially the aquatic ecosystem. The use of microorganisms has been reported to be effective approach for remediation. Five bacterial isolates with the capability of decolourizing textile dyes were isolated from textile effluent and identified as Bacillus thuringiensis (Isolate A2, B6), Bacillus badius (Isolate B5, B9), Bacillus aneurinolyticus (Isolate C2) by different morphological, physiological and biochemical tests. Physicochemical parameters such as temperature, pH and inoculum concentration were optimized for the decolourization process. The optimum temperature, pH and inoculum size for the decolourization of three experimental dyes (Novacron Orange FN-R, Novacron Red FN-R, Terasil Green) were found 30 to 35 °C, 7.0 to 8.0, and 10% (v/v) respectively. The selected bacterial isolates showed different decolourization activities for three experimental dyes. The isolate B5 (Bacillus badius) decolourized 98% of initially added Terasil Green after 48 h of incubation at 35 °C in neutral pH. The present study suggests that the isolated Bacillus sp. can be utilized to treat reactive dyes containing waste water.
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