Biodecolorization of brilliant green carpet industry dye using three distinct Pleurotus spp.

Abstract

Biodecolorization potentials of three distinct white-rot fungi including Pleurotus florida (PF), Pleurotus eryngii (PE) and Pleurotus sajor-caju (PS) were assessed both in liquid and on solid media supplemented with carpet industry dye brilliant green. All three fungi produced laccase and peroxidase enzymes. The decreasing order of laccase production was achieved as 388 > 334 > 301 IU/mL in cultures of PF > PE > PS during 15th, 20th and 17th days, respectively, while, decreasing order of peroxidase production was as 72 > 64 > 55 IU/mL in PF > PE > PS on 15th day of PF, PS cultures and on 20th day of PE, respectively. All tested fungi very efficiently degraded different levels (2, 4, 6 and 8%; w/v) of highly complex synthetic brilliant green dye to colorless. However, PF was found to be best amongst the three species tested on solid and in liquid media. In comparison to higher concentrations, lower concentrations of dye were rapidly biodecolorized by all fungal strains. The order of highest biodecolorization potentialities was recorded as 99 > 91 > 83% by PF > PE > PS, respectively with 2% (w/v) of dye under submerged conditions. While, experimental sets with 4% (w/v) dye were found as the second most rapidly biodecolorized sets, that resulted in 90 > 78 > 70% by PF > PE > PS strains respectively. Based on the findings of biodecolorization potentialities PF was most efficient fungus as compared with other fungi by degrading 99% of the 2% (w/v) dye. Therefore, PF can be subjected in the eco-friendly and cost-effective industrial effluent cleanup processes for the environmental sustainability.