Abstract
Saccharomyces cerevisiae cells ionically adsorbed onto hexamethylene-diamine-treated bagasse (maximum cell loading 0.410 g cells per gram of carrier, dry basis) were used in a packed bed bioreactor at 30 ± 1 °C with sucrose medium (pH 5.0). The dynamic behaviour resulting from perturbations in feed sugar concentration and feed flow rate was studied during the continuous bioconversion of sugar to ethanol. The time-dependent partial differential equations were solved numerically by the Crank-Nicolson method and the experimental data fit well with those computed from the model. The RNA and total protein levels and alcohol dehydrogenase enzyme activity during the transient phase were monitored in a continuous stirred tank bioreactor (free cells) and their levels provide a reasonable explanation of the experimentally observed behaviour.
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