Bioconversion of pretreated wheat straw to ethanol by Monascus purpureus CBS 109.07 and Fusarium venenatum ATCC 20334 using simultaneous saccharification and fermentation

David Yudianto,Claes Niklasson,Mohammad J Taherzadeh,Chusnul Hidayat,Patrik Lennartsson,Ellyas Alga Nainggolan,Ria Millati

doi:10.13057/biodiv/d200817

Abstract

Abstract. Yudianto D, Nainggolan EA, Millati R, Hidayat C, Lennartsson P, Taherzadeh MJ, Niklasson C. 2019. Bioconversion of pretreated wheat straw to ethanol by Monascus purpureus CBS 109.07 and Fusarium venenatum ATCC 20334 using simultaneous saccharification and fermentation. 20: 2229-2235. Fractions of sulfuric acid-pretreated wheat straw, i.e. solid, liquid, and a mixture of liquid and solid were used as substrates in simultaneous saccharification and fermentation (SSF) process to produce ethanol. The bioconversion was performed by Monascus purpureus CBS 109.07 and Fusarium venenatum ATCC 20334. The highest ethanol yields from solid, liquid and a mixture of solid and liquid fractions by M. purpureus CBS 109.07 were 0.36, 0.41, and 0.37 g/g glucose, respectively. The corresponding values by F. venenatum ATCC 20334 were 0.21, 0.54, 0.35 g/g glucose, respectively.

Highlights

Wheat is one of the major crops in the United Kingdom (UK), and according to a report in 2009, the annual wheat straw yield was estimated to be between 8 and 10 million tons (Brander et al 2009)
Cellulase complex can hydrolyze cellulose into monomeric sugars. These monomeric sugars can be converted to ethanol by simultaneous saccharification and fermentation (SSF) method, for example by M. purpureus CBS 109.07 and F. venenatum ATCC 20334
The results showed that M. purpureus has the capability as saccharification agent and ethanol producer

Summary

Introduction

Wheat is one of the major crops in the United Kingdom (UK), and according to a report in 2009, the annual wheat straw yield was estimated to be between 8 and 10 million tons (Brander et al 2009). One of potential usages of wheat straws is by converting it to more value-added products such as ethanol. The contents of glucan and xylan in wheat straw are approximately 36.3% and 19%, respectively, and have the potential to be converted to ethanol (Kootstra et al 2009). The basic steps of producing ethanol from lignocellulosic materials are pretreatment, hydrolysis, and fermentation. Cellulase complex can hydrolyze cellulose into monomeric sugars These monomeric sugars can be converted to ethanol by simultaneous saccharification and fermentation (SSF) method, for example by M. purpureus CBS 109.07 and F. venenatum ATCC 20334. The results showed that M. purpureus has the capability as saccharification agent and ethanol producer. M. purpureus CBS 109.07 and F. venenatum ATCC 20334 are still considered as new strains especially for ethanol production from lignocellulose

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Results