Abstract
Menaquinone (MK) has important applications in the pharmaceutical and food industries. To increase the production rate (QP) of MK-4, we developed a straightforward biotransformation method for MK-4 synthesis directly from its precursors 1,4-dihydroxy-2-naphthoate (DHNA) and farnesol using whole cells of genetically engineered Elizabethkingia meningoseptica. Results showed that MK-4 can be produced directly from farnesol and DHNA using both free and immobilized FM-D198 cells. MK-4 yield peaked at 29.85 ± 0.36mg/L in the organic phase and 24.08 ± 0.33mg/g DCW after 12h of bioconversion using free cells in a two-phase conversion system. MK-4 yield reached 26.34 ± 1.35mg/L and 17.44 ± 1.05mg/g DCW after 8h using immobilized cells. Although this yield was lower than that using free cells, immobilized cells can be re-used for MK-4 production via repeated-batch culture. After ten batch cultures, efficient MK-4 production was maintained at a yield of more than 20mg/L. After optimizing the catalysis system, the MK-4 yield reached 26.91 ± 1.27mg/L using the immobilized cells and had molar conversion rates of 58.56 and 76.90% for DHNA and farnesol, respectively.
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