Abstract
The Δ 1-dehydrogenation of 6-α-methyl-hydrocortisone-21-acetate in an organic-aqueous two-liquid-phase system using free Arthrobacter simplex cells was studied. Chloroform, toluene, and n-octan-1-ol were assayed for steroid solubility and biocompatibility, the latter emerging as the most appropriate solvent. Addition of surfactants (a commercial-grade lecithin, phosphatidylcholine, and phosphatidyl-ethanolamine) led to increased initial reaction rates but did not significantly change the final conversion yields. First-order apparent kinetics was observed in a menadione-saturated system for global substrate concentrations under 10 m m. Maintenance stability was considerably increased when the temperature was lowered from 30 to 25°C. However, operational stability levels were unsufficient for biocatalyst reuse in the tested reactional conditions.
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