Abstract
Objective: The main objective of this study was to determine the effect of biocontrol Trichoderma species agent against Fusarium oxysporum in Solanum lycopersicum L.
 Methods: Genus Trichoderma effective biocontrol agent against fungal, bacterial pathogen. The fungal isolates Trichoderma viride (Tr01), Trichoderma harzianum (Tr02), and F. oxysporum (Fu04) were also identified through morphological characterization observed under a light microscope (10x×40x). The isolates (Tr01, Tr02, Fu04) were confirmed through genetic DNA isolation and polymerase chain reaction analysis. After that pot culture study was conducted to test the antagonistic potential (Tr01, Tr02) and against (Fu04). Seeds of tomato (S. lycopersicum L.) were used in this experiment; treatment such as control, Tr01, Tr02, Fu04, Tr01+ Fu04, and Tr02+ Fu04 was given to the seeds sown in pots and maintained in appropriate distances.
 Results: In pot culture after 45th day after inoculation of bioinoculated plant (Tr02), significantly enhances plant length, fresh weight, dry weight, chlorophyll content, nitrogen content, NPK content, and soil microbial population.
 Conclusion: In this experiment, Trichoderma spp. to control plant pathogen and improve yield and quality of crop. Fungal species belonging to the genus Trichoderma act as a biological agent. Bioinoculated plant (Tr02) significantly enhances plant growth ability to with stand under nutrient deficient conditions.
Highlights
Soil microorganisms are usually present in numerous numbers near plant roots, where more content of organic compounds liberated by the root
The species of infected tissue were placed on potato dextrose agar (PDA) medium was inoculated at 22±2°C 7 days
Diluted samples were placed on PDA medium colony that produced on the light green and dark green in color
Summary
Soil microorganisms are usually present in numerous numbers near plant roots, where more content of organic compounds liberated by the root. Soil-borne pathogens attack a wide range of susceptible plants and cause telluric diseases such as seed decay, damping-off, root rot, and blights [3]. Many researches in the literature recognized tremendous antagonistic profiles of different Trichoderma spp. against soil-borne pathogens. These Trichoderma are used as biological control agents against fungal phytopathogens [4]. It is an important nursery-based vegetable crop cultivated for its fleshy fruits. Tomato is used to produce soup, juice, ketchup, puree, paste, and powder. F. oxysporum is a soil-borne fungal pathogen that infects plants through roots at all stages of plant growth, causes major economic losses by inducing necrosis, and wilting symptoms in many crop plants. The disease causes great losses, especially on the susceptible varieties of tomato, especially when soil and air temperature are rather high during the warm seasons [7]
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