Biocontrol potential of three novel Trichoderma strains: isolation, evaluation and formulation.

A K Mukherjee,P K Mukherjee,A Sampath Kumar,S Kranthi

doi:10.1007/s13205-013-0150-4

A K Mukherjee, P K Mukherjee + Show 2 more

Open Access

https://doi.org/10.1007/s13205-013-0150-4

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Journal: 3 Biotech	Publication Date: Jun 30, 2013
Citations: 37	License type: CC BY 2.0

Affiliation: Central Institute for Cotton Research

Abstract

We have isolated three novel strains of Trichoderma (two T. harzianum and one T. atroviride) from wild mushroom and tree bark, and evaluated their biocontrol potential against Sclerotium delphinii infecting cultivated cotton seedlings. T. harzianum strain CICR-G, isolated as a natural mycoparasite on a tree-pathogenic Ganoderma sp. exhibited the highest disease suppression ability. This isolate was formulated into a talcum-based product and evaluated against the pathogen in non-sterile soil. This isolate conidiated profusely under conditions that are non-conducive for conidiation by three other Trichoderma species tested, thus having an added advantage from commercial perspective.

Highlights

Trichoderma spp. are widely used as commercial biofungicides all over the world (Harman 2006; Harman et al 2004; Howell 2006; Lorito et al 2010; Schuster and Schmoll 2010; Shoresh et al 2010; Verma et al 2007)
Soil/rhizosphere has been classically viewed as the main habitat of Trichoderma, even though the maximum diversity of this species occurs aboveground e.g., on tree bark and wild mushrooms, and mycotrophy is viewed as the ancestral trait of this genus (Druzhinina et al 2011)
We have isolated three novel Trichoderma strains from wild mushroom and tree bark and evaluated their potential as biocontrol agents

Summary

Introduction

Trichoderma spp. are widely used as commercial biofungicides all over the world (Harman 2006; Harman et al 2004; Howell 2006; Lorito et al 2010; Schuster and Schmoll 2010; Shoresh et al 2010; Verma et al 2007). In India alone, more than 250 commercial formulations are available (Singh et al 2012), but almost all of them are based on. K. Mukherjee Central Rice Research Institute, Cuttack, Odisha, India a single strain of T. viride (recently reclassified as T. asperelloides; Mukherjee et al 2013b), isolated from rhizosphere (Sankar and Jeyarajan 1996). Soil/rhizosphere has been classically viewed as the main habitat of Trichoderma, even though the maximum diversity of this species occurs aboveground e.g., on tree bark and wild mushrooms, and mycotrophy is viewed as the ancestral trait of this genus (Druzhinina et al 2011). We have isolated three novel Trichoderma strains from wild mushroom and tree bark and evaluated their potential as biocontrol agents. We have developed a formulation product based on the most effective strain and evaluated this formulation as seed treatment for suppression of seed and root rot of cotton caused by Sclerotium delphinii, an emerging pathogen of cultivated cotton (Mukherjee et al 2013a)

Results and discussion

Experimental procedure

Evaluation of TrichoCASH in green house