Biocontrol Potential of Neem leaf Extract on Mortality of Meloidogyne incognita

Abstract

Root-knot nematode Meloidogyne incognita is one of the widely distributed in India and most economically damaging genera in vegetable crops. Plant products are the safer alternative approaches to control root knot nematodes. An experiment was carried out in vitro to test the Neem leaf extract of aqueous, acetone and methanol extract. Different concentrations of shade-dried leaves of Neem were tested on mortality of Meloidogyne incognita juveniles. Increase in concentration and exposure period resulted in increased mortality rate of Juveniles of M. incognita. Methanol Neem leaf extract (5 ml concentration) proved to be the most effective among used aqueous, acetone and methanol neem leaf extract . After 48 hours exposure aqueous and methanol leaves extract showed 100% mortality while acetone neem leaves extract showed 100% mortality after 72 hours exposure of 5 ml concentration. Neemleaves 0.25 ml con. was the least effective among all con.