Abstract
Mango is an important tropical fruit in the world. Mango anthracnose is one of the most severe postharvest diseases caused by Colletotrichum gloeosporioides . Biocontrol by actinomycetes is considered as a promising strategy. Here, 67 actinomycetes were isolated from mangrove forest soils. A strain labelled with HSL-9B showed 63.21% of the inhibition ratio against C. gloeosporioides and broad-spectrum antifungal activities against the selected seven postharvest pathogens. The strain was assigned to Streptomyces malaysiensis based on the phenotypic, biochemical and whole-genomic profiles . Strain HSL-9B extracts significantly reduced the disease severity of mango anthracnose in vivo . 8 × EC 50 of extracts completely inhibited the infection of pathogen during the entire storage process. Total soluble sugar contents on harvested mangoes were effectively kept. 4 × EC 50 of extracts obviously inhibited spore germination and mycelial growth of C. gloeosporioides , resulting in mycelial rupture and cell ultrastructure destroying. The biosynthesis gene clusters (BGC) of secondary metabolites involved in antagonism were identified by compared with the whole-genome sequencing of strain HSL-9B. Fifteen compounds in strain HSL-9B extracts were obtained using the gas chromatography-mass spectrometry (GC-MS). 12-methyl-tridecanoic acid was one of dominant compounds identified in extracts. Hence, S. malaysiensis HSL-9B is an important bioresource for exploring novel natural products to manage postharvest disease. • Streptomyces strain with strong antifungal activities was isolated and identified. • Strain HSL-9B inhibited mango decay and maintained its quality during postharvest. • Extracts damaged the cell integrity and inhibited spore germination of pathogen. • Biosynthetic gene clusters were found in the sequencing genome of strain HSL-9B. • Several secondary metabolites were identified in strain HSL-9B extracts.
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