Abstract
Bioconjugates made up of an enzyme and gold nanorods (GNRs) were fabricated by electrostatic interactions (layer-by-layer method, LBL) between anionic glucose oxidase (GOD) and positively charged GNRs. The assembled processes were monitored by UV–Vis spectra, zeta potential measurements, and transmission electron microscopy. The enzyme activity assays of the obtained bioconjugates display a relatively enhanced thermostability behavior in contrast with that of free enzyme. Free GOD in solution only retains about 22% of its relative activity at 90 °C. Unexpectedly, the immobilized GOD on GNRs still retains about 39.3% activity after the same treatment. This work will be of significance for the biologic enhancement using other kinds of anisotropic nanostructure and suggests a new way of enhancing enzyme thermostability using anisotropic metal nanomaterials.
Highlights
Considerable effort has been devoted to the study of gold nanoparticles with variable size and shape due to their unique geometry-dependent optical, electronic, and catalytic properties in electronics and optics [1,2,3,4,5], in the fields of biotechnology and nanotechnology [6,7,8]
The bioconjugates of glucose oxidase (GOD) and gold nanorods (GNRs) were fabricated using the electrostatic interaction between GOD and cationic polyelectrolytecoated GNRs, as shown in Scheme 1
The GNRs used in this study were prepared by the seed-mediated growth method in cetyltrimethylammonium bromide (CTAB) surfactant solution [26] and were positively charged due to the Scheme 1 Schematic illustration of the fabrication of the GOD/GNR bioconjugates coating of the CTAB bilayer
Summary
Considerable effort has been devoted to the study of gold nanoparticles with variable size and shape due to their unique geometry-dependent optical, electronic, and catalytic properties in electronics and optics [1,2,3,4,5], in the fields of biotechnology and nanotechnology [6,7,8]. The thermostability of the GNR/GOD bioconjugates was dramatically enhanced, and even at 90 °C, its relative activity still remained at about 39.3%.
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