Biocompatible 3D Matrix with Antimicrobial Properties.

Alberto Ion,Dragoș Rădulescu,Alexandru Grumezescu,Ecaterina Andronescu,Mariana Chifiriuc,Madalina Albu,Marius Rădulescu,Alina Holban,Florin Iordache,Horia Maniu,Adrian Surdu,Bogdan Vasile

doi:10.3390/molecules21010115

Alberto Ion, Dragoș Rădulescu + Show 10 more

Open Access

https://doi.org/10.3390/molecules21010115

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Abstract

The aim of this study was to develop, characterize and assess the biological activity of a new regenerative 3D matrix with antimicrobial properties, based on collagen (COLL), hydroxyapatite (HAp), β-cyclodextrin (β-CD) and usnic acid (UA). The prepared 3D matrix was characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Microscopy (FT-IRM), Transmission Electron Microscopy (TEM), and X-ray Diffraction (XRD). In vitro qualitative and quantitative analyses performed on cultured diploid cells demonstrated that the 3D matrix is biocompatible, allowing the normal development and growth of MG-63 osteoblast-like cells and exhibited an antimicrobial effect, especially on the Staphylococcus aureus strain, explained by the particular higher inhibitory activity of usnic acid (UA) against Gram positive bacterial strains. Our data strongly recommend the obtained 3D matrix to be used as a successful alternative for the fabrication of three dimensional (3D) anti-infective regeneration matrix for bone tissue engineering.

Highlights

Bone cancers, such as osteosarcoma have a great incidence rate for both elderly and patients younger than 20 years old [1]
CDs have been included in the obtained composites as a delivery system for usnic acid, known
Our results demonstrate that the stimulation of proliferation is proportional with the amount of hydroxyapatite, included in the matrix, the greatest cell proliferation is proportional with the amount of hydroxyapatite, included in the 3D matrix, the viability viability rate being observed in the case

Summary

Introduction

Bone cancers, such as osteosarcoma have a great incidence rate for both elderly and patients younger than 20 years old (approximately 5.0 per million/year) [1]. Patients with implanted prostheses present a higher risk to develop severe infections produced by microorganisms that adhere to inserted devices, colonize the surface and form biofilms [8,9]. Due to their architecture, microbial biofilms cause chronic infections because they show increased tolerance to antibiotics and disinfectants, as well as resisti phagocytosis and other components of the body’s defense system [10,11]

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