Biocompatibility Through Cell Attachment and Cell Proliferation Studies of Nylon 6/Chitosan/Ha Electrospun Mats

Abstract

Novel cefixime loaded chitosan/HA/Nylon 6 electropsun mats were prepared with excellent swelling properties and were tested for cell attachment and cell proliferation. FTIR spectra shows that hydrogen bonding is developed between composite fibers, as pronounced shape change could be seen in amide I and II peaks. SEM analysis displayed completely different morphology (fiber diameters, general appearance, pore-size and shape) of composite fibers as compared to N6 fibers. Composite fibers showed high thermal stability in thermal gravimetric analysis. The ultimate tensile strength of fiber films was around 4.45 MPa. Both composite and Nylon 6 fibers demonstrated sustained drug release up to 24 h. Although the composite and control (Nylon 6) fibers, both, provided compatible favorable environment for the osteoblast cells, the composite fibers provided a better suited environment for the osteoblast cells differentiation and in parallel supporting cellular population also composite fibers exhibited superior swelling properties than the control which in turn complement the healing properties of wound dressings.