Biocompatibility of quantum dots (CdSe/ZnS ) in human amniotic membrane-derived mesenchymal stem cells in vitro

Gongping Wang,Xiaoshan Feng,Caie Wang,Guangwei Zeng,Fangxia Guan,Huasheng Wang,Bo Yang,Dongpeng Li

doi:10.5507/bp.2014.045

Abstract

Amniotic membrane-derived mesenchymal stem cells (hAM-dMSCs) are a potential source of mesenchymal stem cells which could be used to repair skin damage. The use of mesenchymal stem cells to repair skin damage requires safe, effective and biocompatible agents to evaluate the effectiveness of the result. Quantum dots (QDs) composed of CdSe/ZnS are semiconductor nanocrystals with broad excitation and narrow emission spectra, which have been considered as a new chemical and fluorescent substance for non-invasively labeling different cells in vitro and in vivo. This study investigated the cytotoxic effects of QDs on hAM-dMSCs at different times following labeling. Using 0.75, 1.5 and 3.0 μL between quantum dots, labeled human amniotic mesenchymal stem cells were collected on days 1, 2 and 4 and observed morphological changes, performed an MTT cell growth assay and flow cytometry for mesenchymal stem cells molecular markers. Quantum dot concentration 0.75 μg/mL labeled under a fluorescence microscope, cell morphology was observed, The MTT assay showed cells in the proliferative phase. Flow cytometry expression CD29, CD31, CD34, CD44, CD90, CD105 and CD106. Within a certain range of concentrations between quantum dots labeled human amniotic mesenchymal stem cells has good biocompatibility.

Highlights

The skin undergoes a cycle of growth and degeneration to ensure the maintenance and homeostasis of adult skin
Quantum dots (QDs) for imaging by fluorescence microscopy QDs emit a red color upon excitation with a single wavelength of light at 570 nm using a fluorescence microscope (DMIL; Leica, Germany). (Fig. 1 A)
Effects of QD-labeling on hAM-dMSCs Under different QD concentrations, QDs could be detected on the plasma membrance of hAM-dMSCs following different QD-labeling times

Summary

Introduction

The skin undergoes a cycle of growth and degeneration to ensure the maintenance and homeostasis of adult skin. Amniotic membrane-derived mesenchymal stem cells (hAM-dMSCs), with low immunogenicity and anti-inflammatory properties, have attracted attention as a novel source of stem cells, providing a potential source of mesenchymal stem cells to repair skin damage. Such repair requires the use of safe, effective and biocompatible agents to evaluate the effectiveness of the result. Using 0.75, 1.5 and 3.0 μL between quantum dots, labeled human amniotic mesenchymal stem cells were collected on days 1, 2 and 4 and observed morphological changes, performed an MTT cell growth assay and flow cytometry for mesenchymal stem cells molecular markers. Within a certain range of concentrations between quantum dots labeled human amniotic mesenchymal stem cells has good biocompatibility

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Conclusion