Abstract
Seeding cells and scaffolds play pivotal roles in bone tissue engineering and regenerative medicine. Wharton's jelly-derived mesenchymal stem cells (WJCs) from human umbilical cord represent attractive and promising seeding cells in tissue regeneration and engineering for treatment applications. This study was carried out to explore the biocompatibility of scaffolds to seeding cells in vitro. Rod-like nano-hydroxyapatite (RN-HA) and flake-like micro-hydroxyapatite (FM-HA) coatings were prepared on Mg-Zn-Ca alloy substrates using micro-arc oxidation and electrochemical deposition. WJCs were utilized to investigate the cellular biocompatibility of Mg-Zn-Ca alloys after different surface modifications by observing the cell adhesion, morphology, proliferation, and osteoblastic differentiation. The in vitro results indicated that the RN-HA coating group was more suitable for cell proliferation and cell osteoblastic differentiation than the FM-HA group, demonstrating better biocompatibility. Our results suggested that the RN-HA coating on Mg-Zn-Ca alloy substrates might be of great potential in bone tissue engineering.
Highlights
Seeding cells and scaffolds play pivotal roles in bone tissue engineering and regenerative medicine
Our results demonstrated that the Rod-like nano-hydroxyapatite (RN-HA) coating enhanced the adhesion and proliferation of Wharton’s jelly-derived mesenchymal stem cells (WJCs), which are in vitro noncytotoxic and biocompatible to cells
These findings indicated that the RN-HA coating induced the deposition of calcium phosphate, demonstrating better biocompatibility
Summary
Seeding cells and scaffolds play pivotal roles in bone tissue engineering and regenerative medicine. Wharton’s jelly-derived mesenchymal stem cells (WJCs) from human umbilical cord represent attractive and promising seeding cells in tissue regeneration and engineering for treatment applications. WJCs were utilized to investigate the cellular biocompatibility of Mg-Zn-Ca alloys after different surface modifications by observing the cell adhesion, morphology, proliferation, and osteoblastic differentiation. Seeding cells and scaffolds serving as matrices for tissue formation play pivotal roles in bone tissue engineering and regenerative medicine. WJCs were isolated and characterized, and rod-like nano-hydroxyapatite (RN-HA) and flake-like microhydroxyapatite (FM-HA) coatings were prepared on MgZn-Ca alloy substrates using MAO and electrochemical deposition (ED). We analyzed the biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human WJCs in vitro, which may provide an important reference for clinical applications
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