Biocompatibility of labeled green fluorescent protein skeletal muscle satellite cells grown on polyglycolic acid fibers

Abstract

Objective To establish a method of isolation and culture of the rat skeletal muscle satellite cells. To investigate the feasibility of in vitro constructing cell-polyglycolic acid (PGA) complex from skeletal muscle satellite cells seeded on PGA. Methods Primary skeletal muscle satellite cells were harvested by enzyme. After series culture and expansion in vitro, Immunohistochemistry staining was carried out to detect the immunophenotypes of skeletal muscle satellite cells. After skeletal muscle satellite cells were transfected by lentiviral vector carrying green fluorescent protein (GFP), skeletal muscle cell-PGA complex was constructed by seeding transfected skeletal muscle satellite cells on PGA fibers. The cell-PGA complex was examined by light microscope and scanning electron microscope (SEM). Results Immunohistochemistry staining showed that skeletal muscle satellite cells expressed Desmin and Vimentin. Microscopic observation showed the production of rich extracellular matrices by transfected skeletal muscle satellite cells was observed in the cell-scaffold construct, SEM observations showed that the secreted matrices filled the space between the PGA fibers. Conclusion Transfected skeletal muscle satellite cells have good biocompatibility with PGA. Imaging with expression of GFP facilitates the research on skeletal muscle satellite cells application in tissue engineering in vivo. Key words: Skeletal muscle satellite cells; Green fluorescent protein; Reconstruction; Tissue engineering