Abstract
Biodentine™ is a tricalcium silicate-based cement material that has a great impact on different biological processes of dental stem cells, compared to other biomaterials. Therefore, we aimed to investigate the optimum biocompatible concentration of Biodentine™ with stem cells derived from periodontal ligament (hPDLSCs) by determining cell proliferation, cytotoxicity, migration, adhesion and mineralization potential. hPDLSCs were treated with Biodentine™ extract at different concentrations; 20, 2, 0.2 and 0.02 mg/mL. Cells cultured without Biodentine™ were used as a blank control. The proliferation potential of hPDLSCs was evaluated by MTT viability analysis for 6 days. Cytotoxicity assay was performed after 3 days by using AnnexinV/7AAD. Migration potential was investigated by wound healing and transwell migration assays at both cellular and molecular levels. The expression levels of chemokines CXCR4, MCP-1 and adhesion molecules FGF-2, FN, VCAM and ICAM-1 were measured by qPCR. The communication potentials of these cells were determined by adhesion assay. In addition, mineralization potential was evaluated by measuring the expression levels of osteogenic markers; ALP, OCN, OPN and Collagen type1 by qPCR. Our results showed significant increase in the proliferation of hPDLSCs at low concentrations of Biodentine™ (2, 0.2 and 0.02 mg/mL) while higher concentration (20 mg/mL) exhibited cytotoxic effect on the cells. Moreover, 2 mg/mL Biodentine™ showed a significant increase in the migration, adhesion and mineralization potentials of the derived cells among all concentrations and when compared to the blank control. Our findings suggest that 2 mg/mL of Biodentine™ is the most biocompatible concentration with hPDLSCs, showing a high stimulatory effect on the biological processes.
Highlights
Cemented root filling materials provide apical sealing which prevents the transfer of bacterial products and other irritants between root canals and the surrounding periapical tissues
Our findings suggest that 2 mg/mL of BiodentineTM is the most biocompatible concentration with human periodontal ligament stem cells (hPDLSCs), showing a high stimulatory effect on the biological processes
A significant increase in the proliferation rate of hPDLSCs was observed when cells were treated with the lower concentrations of BiodentineTM (2, 0.2 and 0.02 mg/mL) (p < 0.05)
Summary
Cemented root filling materials provide apical sealing which prevents the transfer of bacterial products and other irritants between root canals and the surrounding periapical tissues. These materials must be biocompatible with periradicular tissues [1,2,3]. Calcium hydroxide has been the standard material to maintain pulp vitality ever since 1928, due to the capability to form tertiary dentine. Calcium hydroxide was later replaced by MTA (mineral trioxide aggregate). MTA is a mixture of salts containing dicalcium and tricalcium silicate, calcium sulfate dehydrate, tricalcium aluminate and bismuth oxide [4]. MTA is characterized as a superior root filling
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
