Abstract
This study evaluated the performance of an easy-cultivation device for the mass culture of Alexandrium minutum (A. minutum), a dinoflagellate that produces paralytic shellfish toxins (PSTs). Five culture conditions including three different sizes of containers (250 mL conical flask, 500 mL beaker, and 20 L jar) in two different environments (out-incubator and incubator) were compared in terms of growth and PSTs production. Compared with the incubator environment, the out-incubator environment had more fluctuations in temperature and light intensity. Results showed that the cell densities of A. minutum increased in all groups, especially in the conical flask (I, out-incubator, 6.29×106 cells/mL) and the beaker (IV, incubator, 7.28×106 cells/mL). When cultured in the 20 L jar under out-incubator condition, the algae had the lowest cell density (2.82×106 cells/mL) but the highest toxicity (93.42 ± 2.55×10−6 MU/cell). The negative correlation between average growth rate and single-cell toxicity could be explained by biocompatibility, thereby indicating that the low growth rate led to high toxicity. HPLC-FLD showed that the cellular toxicity increased due to the quantitative increase in GTX1/4, which are the more toxic derivatives. The PSTs types consistently contained GTX1/4 and GTX2/3. The differences in algae growth and toxin-production could be due to changes in bacteria (out-incubator) and CO2 (incubator) with vessel size. The effects of environmental factors on algae are strain specific. The out-incubator device can be applied for large-scale cultivation of A. minutum considering the algae density and toxin-producing ability.
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