Biocompatibility of acellular dermal matrix graft evaluated in culture of murine macrophages

Ana Paula Vendramini,Iracilda Zepone Carlos,Rafaela Fernanda Melo,Rosemary Adriana Chiérici Marcantonio

doi:10.1590/s1678-77572006000200001

Ana Paula Vendramini, Iracilda Zepone Carlos + Show 2 more

Open Access

https://doi.org/10.1590/s1678-77572006000200001

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Abstract

The acellular dermal matrix allograft has been used as an alternative to autogenous palatal mucosal graft. The aim of this study was the evaluation of the biocompatibility of an acellular dermal matrix (AlloDerm®) in culture of macrophages. For hydrogen peroxidase determination we used the method of Pick & Kesari, and the Griess method for nitric oxide determination,. Statistical analysis showed no significant difference (p ≤ 0,05) in the release of nitric oxide and hydrogen peroxide by the macrophages exposed to acellular dermal matrix and the negative control. The results suggest that acellular dermal matrix did not activate the cell inflammatory response.

Highlights

Several techniques have been developed to solve gingival aesthetic problems
This study evaluated the cell inflammatory response of in vitro macrophages in contact with the acellular dermal matrix measuring the release of nitric oxide and hydrogen peroxide
The positive control using zymosan-SIGMA to H O release and LPS-SIGMA to nitric oxide (NO) release showed a high potential of macrophages activation and liberation of H2O2 and NO, respectively

Summary

Introduction

Several techniques have been developed to solve gingival aesthetic problems. The techniques can be grouped as pediculated grafts and autogenous grafts (epithelial free grafts and connective tissue graft)[16,22]. A recent material developed for soft tissue grafting was the acellular dermal matrix (AlloDerm®, Lifecore Biomedical, Oral Restorative Division, Chaska,MN)[1,6,25,30,32].This allograft material has advantages to reduce surgical complications, decrease donor site discomfort and improve esthetic tissue. This graft is obtained from an allograft donor skin and produced by a carefully controlled process that removes the epidermis and dermis cells without altering the extracellular matrix structure, reducing the possible immune response and the transmission of diseases. These cells participate in many aspects of host defense, inflammation and immunity, partly through their ability to undergo adaptive responses to the conditions or stimuli that prevail at sites to which they have been attracted[2]

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