Abstract
BackgroundThe aim of this study was to evaluate the biocompatibility and resorption pattern in three-layer poly (lactide-co-glycolide) (PLGA) membrane according to the concentrations of hyaluronic acid (HA) hydrogel in rabbit calvarial defect model. Four standardized circular defects with 8 mm diameter were created on the four rabbit calvarium. Three-layer PLGA membranes (5% and 10% HA gel) were used as the test groups, both collagen membrane and monolayer PLGA membrane as the control groups.ResultsAfter sacrificing the animals after 4 and 8 weeks, block sections were harvested and histological observation was performed. Pus formation was observed in a site on the three-layer PLGA membranes (with 10% HA gel) of 4 weeks group and initial inflammatory responses were observed on the three-layer PLGA membrane group. However, when compared to both the monolayer PLGA membrane group and collagen membrane group, the HA gel-reinforced three-layer PLGA membrane showed improved cell occlusion and retention period, showing the formation of the capsule-like structure. There was no definite difference between the results of the membranes fabricated with either 5% or 10% HA hydrogel.ConclusionThe HA reinforced three-layer PGLA membrane was retained longer than control group and showed good property in cell occlusion. Future study is under process to improve the inflammatory response of the three layer PLGA membranes, which were observed in this study.
Highlights
The aim of this study was to evaluate the biocompatibility and resorption pattern in three-layer poly (PLGA) membrane according to the concentrations of hyaluronic acid (HA) hydrogel in rabbit calvarial defect model
Clinical observations Inflammatory response was observed on a 10% HA hydrogel reinforced three-layer PLGA membrane in one of 4 weeks group during the healing period
Histologic observations Collagen membrane (Bio-Gide®) In 4 weeks group of collagen membrane, there were dense and neat collagen fibers that run parallel to the bone defect
Summary
The aim of this study was to evaluate the biocompatibility and resorption pattern in three-layer poly (lactide-co-glycolide) (PLGA) membrane according to the concentrations of hyaluronic acid (HA) hydrogel in rabbit calvarial defect model. As the most widely used resorbable membrane, usually have superior biocompatibility and tissue affinity than synthetic membrane due to its excellent biological properties [1,2]. Its resorption rate has been reported to be unpredictable by the due to the hydrophobicity, stiffness and low tissue affinity of the reported PLGA membranes, there were difficulties in its clinical applications. Von Arx et al, [11] by their histological study, reported that foreign body reaction occurred due to the degradation products of PLGA membranes. Complement for the problem was required [12]
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