Abstract

This study was conducted to determine whether nature-derived Reynoutria elliptica extracts exhibit biocompatibility and antimicrobial effects against oral pathogens such as Streptococcus mutans and Candida albicans. Fine particles of Reynoutria elliptica extract were used to probe for biocompatibility and antimicrobial activity toward these pathogens, and results were evaluated with an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay, spectrophotometric growth inhibitory assay, the total number of colony-forming units (CFU), an agar disk diffusion test, and scanning electron microscopy (SEM). In addition, UV/VIS spectroscopy was used to determine the levels of flavonoid and polyphenol in experimental solutions. Several experimental groups showed cell viability higher than 70%, and the antimicrobial activity toward both S. mutans and C. albicans was significantly higher than was that seen for the control group. In CFU and agar disk diffusion tests with C. albicans, increases in the concentration of Reynoutria elliptica extract led to significantly increased antimicrobial effects. Additionally, SEM results showed that Reynoutria elliptica extract changed the morphology and density of S. mutans and C. albicans. The results of this research can be applied to the use of Reynoutria elliptica extracts for the development of oral products that are biologically friendly and can control oral diseases such as dental caries and candida-associated denture stomatitis.

Highlights

  • Since the mouth is in direct contact with the external environment, it is constantly invaded by various microbes and has an environment suitable for bacterial growth and proliferation, both nutritionally and physiologically [1,2]

  • The colony-forming units (CFU) for the 50 μg/mL sample (180.00 ± 15.60) was not significantly different from that of the 100 μg/mL sample (170.25 ± 10.44) (p > 0.05). These results demonstrated that Reynoutria elliptica extract significantly inhibited colony formation with S. mutans and C. albicans

  • S. mutans showed an antibacterial effect with no significant difference between experimental groups, regardless of the concentration of the Reynoutria elliptica extract

Read more

Summary

Introduction

Since the mouth is in direct contact with the external environment, it is constantly invaded by various microbes and has an environment suitable for bacterial growth and proliferation, both nutritionally and physiologically [1,2]. Streptococcus mutans, Prevotella intermedia, Porphyromonas gingivalis, and Candida albicans are among these, and the activities of these microbes in the mouth cause various oral diseases such as tooth decay, periodontal disease, oral candidiasis, and inflammation of the mouth [3,4,5,6]. Inhibition of the activity of these oral pathogens is significant in the prevention and treatment of oral diseases [10]. Streptococcus mutans contributes to the rigid attachment of insoluble glucans to the tooth surface through glucosyltransferase action [11,12]. To prevent tooth caries, the use of antimicrobial materials is required to suppress the growth of S. mutans or to inhibit the attachment of other bacteria [14,15,16].

Objectives
Methods
Results
Discussion
Conclusion
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call