Abstract
BackgroundGold-nanobeacons (Au-nanobeacons) have proven to be versatile systems for molecular diagnostics and therapeutic actuators. Here, we present the development and characterization of two gold nanobeacons combined with Förster resonance energy transfer (FRET) based spectral codification for dual mode sequence discrimination. This is the combination of two powerful technologies onto a single nanosystem.ResultsWe proved this concept to detect the most common fusion sequences associated with the development of chronic myeloid leukemia, e13a2 and e14a2. The detection is based on spectral shift of the donor signal to the acceptor, which allows for corroboration of the hybridization event. The Au-nanobeacon acts as scaffold for detection of the target in a homogenous format whose output capability (i.e. additional layer of information) is potentiated via the spectral codification strategy.ConclusionsThe spectral coded Au-nanobeacons permit the detection of each of the pathogenic fusion sequences, with high specificity towards partial complementary sequences. The proposed BioCode Au-nanobeacon concept provides for a nanoplatform for molecular recognition suitable for cancer diagnostics.Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-016-0192-y) contains supplementary material, which is available to authorized users.
Highlights
Gold-nanobeacons (Au-nanobeacons) have proven to be versatile systems for molecular diagnostics and therapeutic actuators
The formation of the BCR-ABL fusion sequences is dependent on the reciprocal translocation between chromosome 9 and 22, originating the Philadelphia chromosome (Ph + chromosome), which is the molecular hallmark of chronic myeloid leukemia (CML) [19, 20]
The BioCode Au-nanobeacon system relies on the Förster resonance energy transfer (FRET) mediated talk between fluorophores on the hairpin and the acceptor labeled oligonucleotides
Summary
Gold-nanobeacons (Au-nanobeacons) have proven to be versatile systems for molecular diagnostics and therapeutic actuators. We present the development and characterization of two gold nanobeacons combined with Förster resonance energy transfer (FRET) based spectral codification for dual mode sequence discrimination. This is the combination of two powerful technologies onto a single nanosystem. Cordeiro et al J Nanobiotechnol (2016) 14:38 a second oligonucleotide sequence that, in its turn, will hybridize in the vicinity of the donor upon target recognition This event will generate a characteristic FRET signal enabling the evaluation of a sample’s composition in terms of presence/absence of target sequence [15, 16]— spectral codification. The resultant transcripts may be used for evaluating the discrimination capability of the proposed biosensor
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