Abstract
An amphiphilic disulfide-containing polyamidoamine was synthesized by Michael-type polyaddition reaction of piperazine to equimolar N, N′-bis(acryloyl)cystamine with 90% yield. The polycationic micelles (198 nm, 32.5 mV), prepared from the amphiphilic polyamidoamine by dialysis method, can condense foreign plasmid DNA to form nanosized polycationic micelles/DNA polyelectrolyte complexes with positive charges, which transfected 293T cells with high efficiency. Under optimized conditions, the transfection efficiencies of polycationic micelles/DNA complexes are comparable to, or even higher than that of commercially available branched PEI (Mw 25 kDa).
Highlights
Polycationic gene delivery vectors have attracted more and more attention in the past decade because of their versatile chemical structures, ease of preparation, lack of immune response in vivo, etc. [1,2,3,4]
An amphiphilic disulfide-containing polyamidoamine was synthesized by Michael-type polyaddition reaction of piperazine to equimolar N, N0-bis(acryloyl)cystamine with 90% yield
Polymers containing reducible disulfide bonds have been widely applied in biomedical field, such as drug delivery [32,33,34], gene delivery [35,36,37,38,39,40,41,42,43,44,45,46] and layer-by-layer assembly [47–49], we here report the first example of the preparation and application of disulfide-containing cationic polymeric micelles in nonviral gene delivery
Summary
Polycationic gene delivery vectors have attracted more and more attention in the past decade because of their versatile chemical structures, ease of preparation, lack of immune response in vivo, etc. [1,2,3,4]. Polycationic gene delivery vectors have attracted more and more attention in the past decade because of their versatile chemical structures, ease of preparation, lack of immune response in vivo, etc. Various cationic polymers have been applied in in vitro and in vivo gene delivery, including polylysine (PLL) [5, 6], polyethylenimine (PEI) [7, 8], polyamidoamine dendrimer [9,10,11,12], cationic polyester [13, 14], poly(beta-aminoester) [15, 16], polyphosphoester and. Nanoscale Res Lett (2010) 5:1804–1811 of polymer 1 into micelles as efficient gene delivery vectors. Polymers containing reducible disulfide bonds have been widely applied in biomedical field, such as drug delivery [32,33,34], gene delivery [35,36,37,38,39,40,41,42,43,44,45,46] and layer-by-layer assembly [47–49], we here report the first example of the preparation and application of disulfide-containing cationic polymeric micelles in nonviral gene delivery
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