Biochezmical Interfering Factors and Blood Cells Indices

Abstract

Background: The presence of interfering substances is the most common preanalytic factor that can affect the precision and accuracy of Blood Cell Count results. These clinical laboratory interferences are difficult to determine and are largely underestimated. Objectives: This study evaluated the effects of biochemical Interfering factors include hyperglycemia, hyperlipemia, and uremia on blood cells indices including different RBC indices, Plt and WBC count. Therefore, this study was conducted to investigate the potential impact of the increased levels of blood lipids, glucose, and urea on different RBC indices, Plt, and WBC count by automated impedance cell counters. Materials and Methods: This Analytical study performed on 366 individuals with hyperglycemia, hyperlipemia, or uremia and 120 randomly selected healthy participants referring to Imam Hospital of Minoodasht city from February to June 2012. Initially, glucose, triglyceride, and urea levels were measured by Biochemical autoanalyzer and then complete blood cell count was done for each participant. Eventually statistical analysis and comparison between case and control groups were done by SPSS v.16 software. Results: Comparison between case and control groups revealed that uremia caused significant increase in MCV and RDW levels (P < 0.05) and had no effect on MCH level (P > 0.05). Both hyperglycemia and hyperlipemia caused increased in MCV level (P < 0.05). In addition, hyperlipemia increased Hb, Hct, and MCH levels (P < 0.05). Conclusions: According to the results of this study, effect of biochemical interfering factors on test results of impedance cell counter must be considered in clinical laboratories.