Abstract
The needlelike crystals which form in aerated submerged cultures of the corn smut Ustilago zeae (PRL-119) were found to be a D-glucolipid, m.p. 146-7°C, [Formula: see text] (pyridine), which contained one carboxyl group, two ester groups, at least two terminal methyl groups, and two D-glucose residues per mole. On the basis of elementary analyses, neutralization equivalent, and molecular weight estimations, the molecular formula of the material isolated was approximately C37H62−6O17. The substance was not shown to be chemically homogeneous. Conditions used in culturing the fungus in stirred fermentors to produce the crystalline metabolic product, which was termed "ustilagic acid", are described, and a procedure for the isolation of the material is given.
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